The Spanish nurse being treated for ebola says she touched her face with a tainted protective glove after helping treat a man dying from the virus.
Theresa Romero, 40, is in quarantine in a Madrid hospital but told doctors she believes she made the mistake after cleaning up after the man.
Heath officials said she had twice entered the room of Spanish missionary Manuel Garcia Viejo - once to change his incontinence pad and then to retrieve items after he had died.
Mrs Romero is the first person to contract the virus outside Africa.
She earlier told El Mundo she had no idea how she was infected and that she had followed all precautions.
Another three people are also quarantined at the Carlos III hospital, including the woman's husband - who had made a video appeal for authorities not to destroy the couple's dog Excalibur.
But according to unconfirmed reports the animal was euthanised at the veterinary hospital at Madrid's Complutense University on Wednesday.
Some 50 other people - who either had contact with Mrs Romero or treated one of the two missionaries who died at the hospital - are also being monitored.
Spanish leader Mariano Rajoy has called for calm and promised "transparency" over the scare, which has raised questions over whether strict safety rules were properly followed.
As West Africa struggles to cope with a spiralling infection rate, burial teams in Sierra Leone have reportedly gone on strike.
The workers, who arguably have one of the world's most dangerous jobs, complain they have not been paid.
The situation is "very embarrassing", said health ministry spokesman Sidie Yahya Tunis.
He promised that money was available for the workers.
"We haven't been paid for two weeks, so we need our money right now," said one angry worker.
"We don't even care if dead bodies have been littered all over the city - all we want is our money. We've been stigmatised in our communities, so let the government pay us our money."
Sky's Alex Crawford saw first-hand the extreme precautions burial teams in the region have to take as they retrieve corpses.
She said teams in neighbouring Liberia - the worst affected country with more than 2,000 deaths - were overwhelmed, with "not enough hours in the day for them to track down the dead".
Any temporary halt in collection only adds to the risk of further infection because the virus can stay on the bodies, said Sky's Health Correspondent Thomas Moore.
Six hundred people have died from the virus in Sierra Leone, according to the World Health Organisation, and more than 3,400 in total.
The virus has swept through Liberia, Guinea, Sierra Leone and Nigeria, with the World Bank saying the financial impact could hit $32.6bn (£20bn) by the end of the year
In Britain, David Cameron chaired an emergency meeting as four hospitals stand by to handle any UK cases.
The US has ordered security agents at airports and other entry ports to screen arriving travellers for signs of the disease.
Texas ebola patient Thomas Eric Duncan - the first to be diagnosed with the disease in the US - died at the Texas Presbyterian Hospital on Wednesday, officials said.
The UN, meanwhile, has said one of its medical officials in Liberia has tested positive for ebola and is receiving treatment.
The unnamed official is the second member of their mission to contract the virus - the other died on 25 September.
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