April Jones' blood was found on a washing machine belonging to the man accused of her murder, a court has heard.
An intensive forensic search of Mark Bridger's home found a stain matching the five-year-old's profile on the glass door, jurors were told.
Prosecutors said traces of April's DNA were also found in the "front crotch area" of his tracksuit bottoms, as well as on a shower curtain.
Blood stains, which provided a "one in a billion" match to April's DNA, were also found on a wood-burning stove, under the carpet and in the grouting of tiles, Mold Crown Court was told.
Bone fragments thought to be from a human skull were also found in Bridger's house.
Elwen Evans, prosecuting, said that when police went into Bridger's home, half an hour before he was arrested, they did so searching for April.
She said: "When they went in there they stated that the house was uncomfortably hot, that there was a strong smell of detergent, and a smell of cleaning products, air freshener and washed clothes."
The family of April Jones arriving at courtBridger had spent the hours before April disappeared from where she had been playing outside her home in Machynlleth, mid-Wales, on October 1 looking at indecent images on his laptop, the jurors were told.
He had looked at a pornographic cartoon of a young girl gagged and restrained as she was being raped.
Having taken the day off, he also trawled Facebook, looking at pictures of local young girls and sending messages asking women out.
After attending his daughter's school parents evening at around 5.20pm, he approached a couple of girls, aged 8 and ten, who were riding their bicycles on the Bryn y Gog estate.
He asked one of them if they would like a "sleepover" with his daughter. When she declined he drove off.
The next witness to see Bridger told police she saw the defendant's car pull up next to garages on the estate.
Miss Evans said: "April was seen playing nearby. The timing of this sighting of the defendant indicates that the events surrounding the abduction, as we say it was, happened quickly."
The police search for April Jones' body, which has not been foundTen minutes later the Land Rover Discovery was seen by another witness driving past Tuffin's petrol station and then on CCTV heading out of the estate.
The court was also told that the former abattoir worker had made up a "story" that he had killed her in a car accident in a bid to "explain away" his role in the killing.
Bridger told police he had "tried to revive" April after running her over with his car but then drove around with her in the vehicle.
He said that he had drunk so much as he drove around and he could not then remember what had happened to her.
He told police: "I just wish I know what I've done with her, where I've put her."
After waking up the next day, Bridger told police he immediately went to his car to see where April was and then began to search for her.
Miss Evans told the court: "He didn't think he would have burned her or buried her or put her in the river, being a father himself he said he would not have done those things.
He told police: "All I want to say to Paul and Coral is that I'm sorry for what happened and if, in my heart of hearts if I knew where she was, then I would tell them because, first, I could help them lay her to rest."
April Jones went missing on October 1However, prosecutors said there was no evidence of a collision involving his car and say there were no blood stains or signs of a clean-up inside the vehicle.
The court heard that three witnesses saw Bridger carrying a black bin bag in a field near his home the morning after April's disappearance.
Miss Evans said: "We ask you, what was Mark Bridger doing at that location? What was in that black bin bag?"
The court heard that the land around that area was excavated but no trace of April was found.
Yesterday, Miss Evans told the court that Bridger was a "fantasist" who had "a clear interest" in child pornography and child murder cases.
There were images on his laptop of April and her half-sisters, aged 13 and 16, as well as those of murdered young girls including Holly Wells and Jessica Chapman from the Soham murder case, the jury heard.
The court heard that Bridger had denied having a "sexual interest" in children.
He told police that he had an interest in the American serial killer Ted Bundy but that: "I never understood the relation he had with killing girls and having sex with corpses and things like that."
Bridger, 47, of Ceinws, denies abducting and murdering April, and unlawfully disposing of and concealing her body - which has never been found - with intent to pervert the course of justice.
The trial continues.
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